Thainema gen. nov. (Leptolyngbyaceae, Synechococcales): A new genus of simple trichal cyanobacteria isolated from a solar saltern environment in Thailand

Simple trichal types constitute a group of cyanobacteria with an abundance of novel, often cryptic taxa. Here, we investigated material collected from wet surface-soil in a saline environment in Petchaburi Province, central Thailand. A morphological comparison of the isolated strain with similar known species, as well as its phylogenetic and species delimitation analyses based on the combined datasets of other related organisms, especially simple trichal cyanobacteria, revealed that the material of this study represented an independent taxon. Using a multifaceted method, we propose that this material represents a new genus, Thainema gen. nov., belonging to the family Leptolyngbyaceae, with the type species Thainema salinarum sp. nov. This novel taxon shares similar ecological habitats with strains previously placed in the same lineage.


Introduction
Cyanobacteria are ubiquitous microorganisms abundantly found in a wide range of terrestrial and aquatic habitats [1]. These microorganisms played a crucial role in the evolution of life on Earth by producing and releasing oxygen into the atmosphere, starting approximately 2.5 billion years ago [2]. Cyanobacteria are one of the most abundant microorganisms and are adequately adapted to extreme environments, such as solar saltern environments, where salt concentrations are higher than that in seawater [3,4]. Cyanobacteria show high tolerance and adaptability to harsh environmental conditions because of their ability to withstand high osmotic pressure, probably as a result of their long evolutionary history [5,6].
Notwithstanding the ecological and evolutionary importance of cyanobacteria, their systematics has significant gaps. These problems are intensified by cultivation difficulties, reduced sampling from regions outside the temperate zone, and challenging species concepts [7][8][9][10]. On the other hand, cyanobacteria are a monophyletic, but morphologically diverse, group [11,12]. Moreover, the traditional classification of cyanobacteria, based on morphological features, takes no notice of cryptic taxa (e.g., [13,14] Molecular phylogenetic analysis has become a powerful approach in modern taxonomy and has been used to elucidate the evolutionary patterns of cyanobacteria [15][16][17]. Taxonomic delineation of organisms based on the 16S rRNA gene improves their classification and is recognized as the most commonly used molecular marker-based method for the identification of prokaryotes [9,18]. In addition, predictions of the secondary structure of the 16S-23S internal transcribed spacer (ITS) region are regarded as a fundamental tool for the speciation of cyanobacteria [19][20][21][22][23]. However, the identification of many taxa within some cyanobacterial orders remains difficult, and further research is needed to better elucidate the relationships among their members. Although some approaches for species identification are rarely used for cyanobacteria, they are increasingly being used for algae and cyanolichens (e.g., [24,25]). Species delimitation analyses using tree-based and non-tree-based methods have enabled the identification of novel species, and are being utilized as a substantive concept in current systematics [26,27].
Synechococcales is a large, polyphyletic order comprising more than 90 genera of both unicellular and filamentous cyanobacteria; however, most of the families and genera within the order have not been revised by modern phylogenetic studies [9]. For example, Leptolyngbyaceae, one of the largest families in the order Synechococcales, has been investigated more extensively than any other family in this order. Most of the genera in the Leptolyngbyaceae family are monophyletic, with some exceptions that await precise polyphasic analyses (see reviews [9, 14, 19, 28-34]).
A large number of genetic and morphological studies have been conducted to date, with a focus on the taxonomy of Leptolyngbyaceae (e.g., [29,30,35,36]). Li & Li [35] transferred five strains of Planktolyngbya circumcreta to the new genus Limnolyngbya within the Leptolyngbyaceae family. However, within this family, the general systematic patterns of the genera have yet to be clarified. Halomicronema, one of the most well-known filamentous taxa of microbial mats in extreme hypersaline habitats, grows in high salt concentrations ranging from 7% to 15% [37,38]. Halomicronema sp. was morphologically identified by Chatchawan et al. [39] in solar saltern environments. However, some of morphological characters of this strain were not consistent with the type species Halomicronema excentricum Abed et al. [37]; therefore, they suggested molecular analysis for a detailed validation [39].
In this study, we focused on cyanobacterial matter isolated from a manmade solar saltern environment. This material, as cited above, was first reported and morphologically characterized by Chatchawan et al. [39] under the name of Halomicronema sp. We sought to (1) perform additional morphological analysis of this material, including ultrastructure analysis; (2) conduct its molecular characterization as well as an analysis of the phylogenetic relationships of the strains with the other members of the Synechococcales order; and (3) determine the evolutionary lineages and species delimitation of Halomicronema sp. using both tree-and genetic distance-based approaches. Based on the results of this study, we propose that this material is a new genus within the Leptolyngbyaceae family.

Collection site and strain isolation
The source material was collected by Chatchawan et al. [39] from wet soil in shallow evaporation basins in the Ban Laem district, Petchaburi Province, Thailand (13.30 N, 100.07 E), in November-December 2009. After sampling, a portion of soil sample was transferred onto BG-11 agar medium containing different salt concentrations, with the soil spread throughout the medium and the cultures maintained under the following conditions: 25˚C, 12 h light/12 h dark cycle, and 28 μmol�m 2 �s -1 light intensity. The cyanobacterial species were isolated, transferred onto new agar medium, and maintained until monospecific strains were acquired [39]. The strains were maintained as a private collection at the Institute of Botany AS CR, Třeboň, Czech Republic. Subsequently, the strain was deposited into the CCALA culture collection (Třeboň, Czech Republic) under the accession number CCALA 10287. Additionally, the UTEX B SP44 Pseudanabaena galeata strain was obtained from the UTEX culture collection and compared with the material collected in this study.

Microscopic investigation
The morphological characteristics of the isolated cyanobacterial strain were analyzed using the Olympus BX 53 light microscope (LM) and identified according to Komárek & Anagnostidis [40].
To conduct the transmission electron microscopy analysis of the isolated strain, the cells were preserved in a mixture of 2.5% glutaraldehyde and 0.1 M cacodylate buffer, and then washed with the same buffer. Subsequently, the cells were postfixed in 2% osmium tetroxide, dehydrated using an acetone dilution series, and then embedded in Spurr's resin [41]. Thin sections were cut using a diamond knife, placed on Formvar coated grids, contrast-treated with uranyl acetate and lead citrate, and then coated with carbon. The sections were observed with a JEOL 1010 transmission electron microscope (TEM).
Four plasmids were sequenced for strain CCALA 10287, whereas three plasmids were sequenced for strain UTEX SP44. The sequences were submitted to the National Center for Biotechnology Information (NCBI) GenBank database (www.ncbi.nlm.nih.gov) (S3 Table).

Sequence and phylogenetic analyses
Sequences of the cloned PCR products were merged using BioEdit version 7.2.5 [47] and SeqScape version 4.0 (Applied Biosystems), and then compared with sequences available in the NCBI database using BLASTn (http://www.ncbi.nlm.nih.gov/BLAST). The new sequences were aligned to 129 sequences from the NCBI database (S3 Table) using MAFFT version 7 [48], with default settings. Gloeobacter violaceus was designated as an outgroup. An uncorrected pairwise genetic distance (P-distance) for 16S rRNA was estimated with 1,000 bootstrap replicates in MEGA 6 [49] using (100 x (1-genetic distance)). Genetic distance within the members of the newly described genus and between all groups was calculated based on the uncorrected pairwise genetic distance with 1,000 bootstrap replicates using MEGA version 6. The multilocus datasets of 16S rRNA (1,160 bp), rpoC1 (871 bp), and rbcLX (777 bp) genes, and the ITS region (421 bp) were concatenated. PartitionFinder 2.1 [50] was used to recognize the best model of sequence evolution for each partition using the "greedy" algorithm and Bayesian Information Criterion (BIC).
To evaluate the relative support of the branches, Maximum Likelihood (ML) analysis was accomplished using the IQ-TREE web server [51]. A total of 1,000 bootstrap pseudoreplications were run to evaluate the relative support of the branches [52]. Bayesian Inference (BI) analyses involved two runs of eight Markov chains, executed for 60 million generations using default parameters and sampled after every 1,000 generations. The final average standard deviation of split frequencies was <0.01, and the first 25% of the sampled data was discarded as burn-in. Convergences were checked using Tracer version 1.7 [53], and phylogenetic trees were redrawn in FigTree version 1.4.4 [54].

Molecular diagnosis of families
Five conserved regions (helices 18, 20, 23, 27, and 34) of the 16S rRNA gene sequence were selected for family-level identification of the collected material, based on comparisons with the same regions of other related taxa (type species or reference sequences), and two regions (helices 23 and 27) were chosen for secondary structure comparisons [14,55].

Species delimitation
Four species-boundary analyses were performed to evaluate the theoretical species boundaries in the 16S rRNA dataset.
Genetic distance-based and secondary structure analyses. The 'barcoding gap' concept was used to identify the strain at the species level using the 16S rRNA dataset. Automatic Barcode Gap Discovery (ABGD) analysis (http://wwwabi.snv.jussieu.fr/public/abgd/abgdweb. html) was run to detect barcode gaps in the pairwise genetic distance of the dataset. The maximum value of the prior intraspecific divergence was set between 0.001 and 0.01.
The secondary structure of the 16S-23S rRNA ITS region (including D1-D1´and Box-B helices) was folded using the RNA Secondary Structure Prediction Web Server (https://rna. urmc.rochester.edu/RNAstructureWeb), and then redrawn in CorelDRAW Graphics Suite 2018.
Tree-based analyses. If the putative species limits are unknown, the Generalized Mixed Yule Coalescent (GMYC) approach can be used to estimate the species boundaries. The likelihood framework in GMYC allows for statistical inference and hypothesis testing across the entire clade, which is beneficial. The GMYC analyses were carried out using the ape, gee, MASS, paran, and splits packages of the R statistical program. The GMYC approach, which uses an ultrametric gene tree reconstructed in Beast 1.8.2 using a clock model [56], was applied using an uncorrelated lognormal relaxed molecular clock as a model. The BIC, which is implemented in PartitionFinder 2.1.1, was used to find the best substitution model [50]. The analysis was executed under the constant population size coalescent, as the tree prior, and the Ucld. mean parameter was set prior to exponential distribution, with a mean of 10 and an initial value of two independent chains for 600 million generations, with sampling every 10,000 th generation, and with the removal of the first 25% of burn-in trees. Tracer version 1.7 [53] was used to check the outputs for convergence, and TreeAnnotator 1.8.2 was used to generate the consensus tree [56]. The ML of the GMYC model was tested using a likelihood ratio test against a null model that treated the entire tree as a single coalescent (i.e., against a one-species model).
The Bayesian version of Poisson Tree Processes (bPTP) analysis, based on the MrBayes consensus tree, was also run for delimiting species on a rooted phylogenetic tree. The analysis was carried out on the bPTP web server (http://species.h-its.org/ptp/) for 600,000 generations using 0.3 burn-in and 100 thinning, and both Bayesian and ML algorithms for bPTP were considered.

Phylogenetic analysis
A phylogenetic tree was constructed based on a multilocus (16S rRNA, 16S-23S ITS, rpoC1, and rbcLX) dataset of 3,229 bp. The results revealed at least 10 different lineages in the order Synechococcales (Fig 1) Collectively, Thainema gen. nov. and its sister clade were separated from the Acaryochloridaceae, Merismopediaceae, and Thermosynechococcaceae clades with strong support values (BS = 100; PP = 1). The clades mentioned above were separated from the whole dataset with the highest support values (BS = 97; PP = 0.98). The phylogenetic tree showed that Leptolyngbyaceae and Pseudanabaenaceae families exhibit a polyphyletic structure within the order. Tables 1 and 2 show the sequence similarity of the 16S rRNA gene within the Thainema gen. nov. members and between other phylogenetically related groups in the tree, respectively. The highest and lowest values of sequence similarity between groups were estimated to be 93.5% for Trichocoleaceae and 90.2% for Leptolyngbyaceae respectively. The average similarity in the 16S rRNA gene sequences between families was 92.1%.

Species delimitation
The ABGD, bPTP, and GMYC analyses revealed 34, 47, and 54 species, respectively, based on the 16S rRNA sequences; however, 49 and 58 groups were recovered based on the D1-D1´and Box-B helices of the 16S-23S rRNA ITS secondary structure, respectively (Fig 4 and S1 Fig).
The results of phylogenetic and species delimitation analyses revealed four additional strains closely related to Thainema strains: Leptolyngbya sp. UMPCCC 1239, which inhabited the coastal water of Sanibel Island, Florida, USA; Pseudanabaena galeata UTEX SP44, which originated from the pool sediment of Great Salt Plains, Oklahoma, USA; Pseudanabaena sp. YACCYB277, reported from Xinjiang, China; and uncultured Pleurocapsa sp. (Figs 1 and 4). In addition to the high similarity in the 16S rRNA gene sequences between members of Thainema gen. nov. (Table 1), uncultured Pleurocapsa sp. was an airborne organism from Oahu, Hawaii, USA, which grew on both marine and freshwater media; therefore, we could not exclude the possibility that it originated from almost the same type of habitat as the other strains in the new genus.
Only the ABGD analysis revealed the Thainema clade as a single species; however, the 16S rRNA threshold (sequence dissimilarity > 1.3%) provided strong evidence for a different species [58][59][60]. Species delimitation analyses (GMYC and bPTP) identified four and three species, respectively within Thainema gen. nov.  Table 3. Nucleotide variation between families within the order Synechococcales.
Additionally, secondary structures of D1-D1´and Box-B helices in the ITS region of strains CCALA 10287 and UTEX SP44 are depicted in Fig 5. The D1-D1´helices of strains CCALA 10287 and UTEX SP44 were highly similar (61 and 63 bp, respectively). By contrast, the Box-B helices (55 bp) of both strains differed at many positions (Fig 5). Sequences of the 16S-23S ITS region in the other strains were not available for comparison.

Morphological and taxonomic descriptions
The previous description of the strain Thainema, which was introduced as Halomicronema sp.
[39], did not include some of the morphologic features, such as colorless sheath, granules, and the size of trichomes was only approximated. In addition, authors of the cited work decided not to establish a new species. Here, we amend the description of the species below to show its separation from the genus Halomicronema, based on both morphological and molecular

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characterizations. We describe one new genus that includes one new species, whose monophyletic position is strongly supported.

Class Cyanophyceae Order Synechococcales Family Leptolyngbyaceae
Thainema Rasouli-Dogaheh et Hauer gen. nov. Description. Filaments straight or flexuous, mostly bright blue-green or green; no branching, immotile; sheath colorless, sometimes indistinct under the LM; no heterocytes or other special cells present. Trichomes solitary, cylindrical, <3 μm wide, and slightly constricted at the cross-walls. Cells mostly isodiametric or slightly longer than wide, usually with pale-to-shiny granules located at cross-walls; no aerotopes. Terminal cells rounded, without calyptra, and morphologically similar to other cells. Reproduction by trichome fragmentation. Halophilic to halotolerant (Figs 6 and 7).
Etymology. Thai (ไทย) refers to Thailand-country of origin of the type species,-nema (νήμα)-thread (Gr.), refers to appearance of the organism.
Type species. Thainema salinarum Rasouli-Dogaheh et Hauer, sp. nov. Description. Colony pale to bright blue-green. Filaments, 1-3 μm wide. Trichomes mostly straight and slightly constricted, or not constricted at the cross-walls. One trichome present in a sheath with no branching, but the presence of sheaths for some filaments could not be determined with certainty under the LM. Cells longer than wide (2-3.5 μm long), mostly isodiametric (1.5-2 μm long) with pale yellowish-to-golden granules. Reproduction by trichome fragmentation. Aerotopes absent. Terminal cells typically rounded, without calyptra. Necridic cells absent (Fig 6). TEM micrographs demonstrated definite presence of sheaths. Parietal thylakoids (4-5 per cell) were visible in the longitudinal section but were less discernable in the cross-section. Cyanophycin granules were observed among thylakoids. Apical cells were rounded. The cell wall was simple like that of most species in the Leptolyngbyaceae family (Fig 7).
Etymology. Name of the species refers to the original habitat of the material. Diagnosis. According to a recent taxonomic classification [9], Thainema salinarum differs from Halomicronema excentricum morphologically in terms of cell size and the lack of gliding motility and gas vesicles. However, both species share the same ecological habitat and were isolated from benthic microbial mats in a manmade hypersaline environment.

Discussion
The multilocus and 16S rRNA data-based phylogenetic trees revealed a new monophyletic genus, Thainema gen. nov. Our results demonstrate that Thainema gen. nov. is a sister clade of the genera Nodosilinea and Halomicronema, and was previously proposed to be transferred from the Leptolyngbyaceae family to Prochlorotrichacae [14]. It is particularly problematic to delimit a family in prokaryotes based on their morphological characteristics [61,62]. In cyanobacteria, a family has traditionally been described by a set of morphological features, which do not reflect its evolutionary history [9,63]. Diverse morphological autapomorphy was one of

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the greatest restrictions inside several genera within different families, especially in the order Synechococcales. Therefore, taxonomists discovered molecular markers in the amino acid and 16S rRNA gene sequences of organisms, which were shared only by members of a specific population of species, and these shared sequences denote synapomorphies between the common ancestors and all of their descendants [64][65][66][67]. Additionally, the secondary structure of 16S rRNA has been recognized as a useful tool for taxonomic classification in the higher levels of cyanobacteria [55]. According to the latest research (e.g. [14]), measurement of morphological characteristics and determination of 16S rRNA genetic identity cutoff values, synapomorphic nucleotides of the 16S rRNA sequence, and secondary structures of helices 18, 20, 23, 27, and 34 could lead to the family-level identification of a species. Most of the previous studies suggested further division of the Leptolyngbyaceae family [19, 29, 30, 35, 36, 38]. Recently, Mai et al. [14] proposed that the Leptolyngbyaceae should be broken down into four family-level clades (Prochlorotrichaceae, Oculatellaceae, Leptolyngbyaceae, and Trichocoleaceae). In the current study, it was not feasible to use the 16S rRNA genetic identity cutoff values for family delimitation because of high average similarity (92.1%) between genera belonging to different

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families, which is consistent with the result of Mai et al. [14]. However, Thainema gen. nov. shared the highest number of nucleotides with the Acaryochloridaceae family members at conserved sites in the 16s rRNA sequence.
In this study, both 16S rRNA and multilocus datasets revealed the same phylogenetic relationships between clades (Figs 1 and 4), which is consistent with previous studies [14,57,68,69]. Even though previous studies [19,31,32] had only considered the genus Leptolyngbya as a polyphyletic genus within the family Leptolyngbyaceae, our phylogenetic trees revealed the Leptolyngbyaceae family as a polyphyletic group, and this classification is congruent with that of Mai et al. [14], who based their classification on the sequence of rpoC1. However, the family Leptolyngbyaceae has received much greater attention from both traditional and modern taxonomy, than any other families [70]. Therefore, a precise taxonomic analysis was required to re-evaluate its phyletic status. Consequently, we avoided proposing a new family or placing the new genus into the Acaryochloridaceae family; instead, we present Thainema gen. nov as a member of the Leptolyngbyaceae family. Alternatively, we suggest a hybrid approach, which incorporates all previously submitted methods as well as the chemical composition used by Zimba et al. [57], for family-level identification of the Toxifilum genus within the Pseudanabaenaceae family for future studies.
In this work, we sought to identify the molecular and morphological overlap between the CCALA 10287 strain (isolated from wet soil in shallow evaporation basins in Ban Laem district, Petchaburi Province, Thailand [39]) and four other identical strains not related to any of the recognized Leptolyngbyaceae taxa. The public sequence repositories contain a large number of sequences that have either been incorrectly assigned to known organisms or have been assigned a provisional taxonomic designation, which is a threat to cyanobacterial taxonomy. The NCBI Reference Sequences (RefSeq) Database (https://www.ncbi.nlm.nih.gov/refseq/) can be used as a tool for the identification of reference sequences, but it has some limitations. The current version of RefSeq follows neither the International Code of Nomenclature for Algae, Fungi, or Plants (ICN) [71], nor the International Code of Nomenclature of Prokaryotes (ICNP) [72], both of which are used for the classification of cyanobacteria. This problem can lead to incorrect phylogenies or overlooked novel taxa. Despite the vast amount of molecular data available from all over the world, many taxa remain unrecognized. For example, Blank & Hinman [73] isolated 29 strains and performed phylogenetic analyses based on their 16S rRNA gene sequences. Most of the cyanobacterial strains clustered in four large, well-characterized clades, but some strains such as Leptolyngbya sp. UMPCCC 1239 remained separate and were submitted to NCBI as an unclassified sequence. In the current study, we included such sequences (KM218876, MH683727, and KM462585) in our analyses to determine their phylogenetic placement and confirmed that it belongs to a new taxon in the family Leptolyngbyaceae. Thus, our results will assist in reducing the number of sequences with provisional names that belong to currently unknown taxa. Overall, it is highly advisable to utilize as many sequences of type species (according to ICN) or type strains (according to ICNP) and other reference sequences of particular taxa published in their protologues or taxonomic revisions as possible. Such sequences are listed, for example, in CyanoDB 2.0 [70].
In the present study, data available on the origins of the studied strains show an interesting pattern in their geographical distribution. Despite substantial molecular analyses conducted to expand our knowledge of cyanobacterial diversity worldwide in saline, non-planktic habitats, including marine coasts (e.g., [74,75]), mangroves (e.g., [69]), and brackish waters (e.g., [76]), and the high number of publicly available sequences, the distribution of Thainema gen. nov. seems limited to the Northern Hemisphere, notably East Asia (Thailand, this study, red circle on the map; China, accession number: MH683727), the Pacific Region (Hawaii, accession number: KM462585), and North America (strains UMPCCC 1239 and UTEX SP44 [red circle on the map], accession numbers: KM218876 and HQ658458, respectively). This shows that the Baas Becking (1934) hypothesis, i.e., "everything is everywhere, but the environment selects", cannot generally be applied to all microorganisms. We speculate that the taxon originally evolved in North America, and its propagules were then transported by trade winds to Asia. Sherwood et al. [77], who investigated airborne algae on the island of Oahu, Hawaii, and published sequence KM462585, supports this hypothesis. On the generic level, the distribution pattern of cyanobacterial strains is plainly in contrast to that of either the cosmopolitan taxa, such as members of the genus Nostoc Vaucher ex Bornet et Flahault ( [78]: 181), or Myxacorys Pietrasiak et Johansen ([79]: 980), whose distribution is limited to the deserts of the Western Hemisphere. In many cases, the distribution pattern can be skewed by the disproportionate activities of researchers around the world, since studies are often performed in the "favorite research locations" of specific teams. We believe that such a bias is low in saline and nonplanktic habitats.
According to the literature on species concepts [8,80,81], it seems that the classification of genera, at least in certain cases, is more complicated than the separation of different species within the same genus [14]. Furthermore, employing the 16S rRNA gene is not adequate for species delimitation of all genera of the cyanobacteria [31,82]. The 16S-23S ITS region can be better applied to species separation [19-21, 23]. Here, the question that arises is whether all strains in the newly described clade (Thainema) belong to the same species, or whether they should be considered as separate taxa. The main taxonomic approaches used in this study for the classification of Thainema provided consistent morphological, biogeographical, ecological, and molecular data within the genus. Therefore, we followed 16s rRNA-based species delimitation approaches [83][84][85][86][87][88][89] as well as utility of secondary structure in the 16S-23S ITS region [30, 82,90], which both are often used to separate genetically distinct taxa.
Hence, P-distance-based methods, such as ABGD and 16S rRNA sequence similarity, were used in this study. The genetic distance approach has been one of the most commonly used methods for the identification of eukaryotic species [91][92][93]; however, it has also been used for prokaryotes such as cyanobacteria [94]. In this study, both analyses confirmed that the Thainema lineage diverged from other lineages of cyanobacteria (Fig 4, Table 2). In addition, the results of ABGD analysis revealed that the Thainema clade represents a single species; however, considering the length of sequences, the sequence similarity threshold separated the clade into four species. While the ABGD approach is promising, it has certain disadvantages when used for cyanobacterial species delimitation [94]. Our results showed that this method is not well matched with the threshold of similarity (97% or 99%) in genetic distances. Thus, it may not be the best method for determining gaps among multiple species, based on nucleotide substitutions of a single gene [94], especially when the species are genetically polyphyletic [95]. Therefore, employing additional methods for delimiting the species units would lead to a more accurate classification.
In this study, we used tree-based methods (GMYC, bPTP) on the 16S rRNA dataset [96][97][98][99], and found that the results of the similarity method were almost in accord with those of tree-based analyses. Dvořák et al. [100] only used the PTP method, and proved that it could adequately delimit the cyanobacterial species. In the current study, the GMYC approach was more adept at recognizing species than the bPTP and ABGD approaches. This result is consistent with previous findings, which showed that the GMYC method provided more usable taxonomic units than other species delimitation methodologies [91,92,97,99,[101][102][103]. Furthermore, one functional pattern analysis (ITS secondary structure) of the 16S-23S ITS dataset was applied in this study. This analysis clearly represented diagnostic apomorphic characteristics, which were consistent with the 16S rRNA gene-based phylogeny of cyanobacteria [19,20,104]. Unfortunately, we could not gain access to all molecular data on the 16S-23S ITS region. Nevertheless, among the various methods used in this study, the16S-23S ITS secondary structure proved to be the most sensitive tool for separating the different genetic groups of cyanobacteria. Moreover, we did not have morphological data to reliably confirm the existence of more species in the newly described genus. Because of the above-mentioned factors, we hesitate to establish more than one species in the genus based on the current dataset, we also recommend using an integrating species delimitation method and the 16S-23S ITS secondary structure to develop an accurate taxonomic workflow for future research.